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产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteIFN-γFormat96T(8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity< 3.125 pg/mLStandard Curve Range3.125-200 pg/mLAssay Time2 hr 50 minSuitable Sample TypeFor the quantitative determination of human IFN-γ in Cell Culture Supernatants, Plasma, Serum.Sample volume100 uL产品概述（Product Overview）
resDetect™ Human Interferon-γ (IFN-γ) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IFN-γ residues in cell culture supernatants, serum, and plasma. The kit contains GMP Human Interferon-γ (IFN-γ) (ACROBiosystems, cat# GMP-IFGH24) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human IFN-γ.
应用说明（Application）
The Human Interferon-γ (IFN-γ) (Residue Testing)was developed for the detection and quantitative determination of GMP human IFN-γ in samples from CAR-T product preparation processing.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
2-8℃
组分（Materials Provided）
IDComponentsSizeRES028-C01Pre-coated Anti-IFN-γ Antibody Microplate1 plate(8×12 strips)RES028-C02Human IFN-γ Standard20 μgRES028-C03Biotin-Anti-IFN-γ Antibody50 μLRES028-C04Streptavidin-HRP50 μLRES028-C0510xWashing Buffer50 mLRES028-C062xDilution Buffer50 mLRES028-C07Substrate Solution12 mLRES028-C08Stop Solution7 mL原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human IFN-γ. The kit consists of Pre-coated Anti-IFN-γ Antibody Microplate and Human IFN-γ Standard and Biotin-Anti-IFN-γ Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Human IFN-γ as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-IFN-γ Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
质量管理控制体系（QMS）
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
稀释线性（Dilution Linearity）
To assess the linearity of the assay, samples spiked with high concentrations of human IFN-γ, were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
回收率（Recovery）
Three parts of blank serum were added with different concentrations of human IFN-γ and the serum without human IFN-γ was used as background to calculate the recovery rate. The range of the recovery rate is 89.2-116.0%, and the average recovery is 98.3%.
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背景介绍
Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.
Cytokine storm syndromes is an activation cascade of pro-inflammatory cytokines，such as TNF-α，IL-1，IL-6，IL-12，IFN-α，IFN-β，IFN-γ，MCP-1 and IL-8,due to unregulated host immune response system to different stimuli such as infections, malignancy, rheumatoid disorders, drugs, and so on. Interferon-γ (IFN-γ), one of the four subclasses ofinterferons, and is a multipotent protein, acting on many cell types by inducing or inhibiting many cellular functions through direct effects on gene expression.
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