resDetect™ Human Interleukin-1 beta (IL-1 beta) ELISA Kit (Residue Testing)

产品参数（Product Specifications）

产品概述（Product Overview）

Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties; The mature human IL1β shares 96 % amino acid sequence identity with rhesus and 67 % -78 % with canine, mouse and rat IL-1β.

应用说明（Application）

Human Interleukin-1 beta (IL-1 beta) ELISA Kit (Residue Testing) is developed for the detection and quantitative determination of GMP human IL-1 beta in samples from CAR-T product preparation processing.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

组分（Materials Provided）

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human IL-1 beta. The kit consists of Pre-coated Anti-IL-1 beta Antibody Microplate and Human IL-1 beta Standard and Biotin-Anti-IL-1 beta Antibody and Streptavidin-HRP and buffers.

Your experiment will include 6 simple steps:

a) Bring all reagents to room temperature(20℃-25℃) before use.

b) Add your sample to the plate and take the Human IL-1 beta as standard. The samples and standard are diluted by Dilution Buffer.

c) Wash the plate and add the Biotin-Anti-IL-1 beta Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

e) Wash the plate and add TMB.

f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程