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ClinMax™ Mouse IL-1β ELISA Kit

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货号-规格
价格
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CEA-C250-96tests
¥3120.00
合计0件 产品金额¥ 0

产品详情

  • 产品概述(Product Details)

    ClinMax™ Mouse IL-1β ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant mouse IL-1β that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Ready-to-use reagents to save assay time.

  • 应用说明(Application)

    The kit is developed for quantitative detection of natural and recombinant mouse IL-1β in serum, plasma and cell culture supernatants.

    This Assay has been anchored to NlBSC/WHO standards (Code: 93/668), ClinMax ELISA Kit: NIBSC/WHO Mass Conversion Factor is 1.

    It is for research use only.

  • 流程图(Workflow)

    IL-1β Workflow

  • 关键信息(Key Features)

    Assay Type
    Sandwich-ELISA
    Analyte
    Mouse IL-1β
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Mouse
    Sensitivity
    3pg/mL
    Range
    9.4 pg/mL-600pg/mL
    Assay Time
    2.75 hr
    Sample volume
    50 uL
    Sample Type
    Cell Culture Supernatants, Plasma, Serum.
    NIBSC Code
    93/668

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • 存储(Storage)

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 组分(Materials Provided)

    ID
    Components
    Size
    CEA250-C01
    Pre-coated Anti-mouse IL-1β Antibody Microplate
    1 plate(8×12 strips)
    CEA250-C02
    Mouse IL-1β Standard
    6ng×2
    CEA250-C03
    Biotin-Anti-mouse IL-1β Antibody Con. Solution
    100μL
    CEA250-C04
    Biotin-Antibody Dilution Buffer
    8mL
    CEA250-C05
    Streptavidin-HRP Con. Solution
    500μL
    CEA250-C06
    Streptavidin-HRP Dilution Buffer
    15mL
    CEA250-C07
    20× Washing Buffer
    50mL
    CEA250-C08
    Sample Dilution Buffer
    15mL ×2
    CEA250-C09
    Substrate Solution
    12mL
    CEA250-C10
    Stop Solution
    6mL
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

     IL-1β TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of mouse IL-1β is less than 3 pg/mL.

验证
  • 批内差异(Intra-Assay Statistics)

    Ten replicates of each of 3 samples containing different mouse IL-1β concentra-tions were tested in one assay. Acceptable criteria: CV<10%.

     IL-1β INTRA-ASSAY STATISTICS
  • 批间差异(Inter-Assay Statistics)

    3 samples containing different concentrations of mouse IL-1β were tested in in-dependent assays. Acceptable criteria: CV<15%.

     IL-1β INTER-ASSAY STATISTICS
  • 回收率(Recovery)

    Recombinant mouse IL-1β was spiked into 3 mouse serum samples, and then analyzed. The average recovery of IL-1β for serum samples is 98.7%.

     IL-1β RECOVERY

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