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产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteIL-7Format96T(8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity＜6.25pg/mLStandard Curve Range6.25 pg/mL-200 pg/mLAssay Time2 hr 50 minSuitable Sample TypeFor the quantitative determination of human IL-7 in Cell Culture Supernatants, Plasma, Serum.Sample volume100 uL产品概述（Product Overview）
resDetect™ Human Interleukin-7 (IL-7) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IL-7 residues in cell culture supernatants, serum, and plasma. The kit contains GMP human IL-7 (ACROBiosystems, cat# GMP-L07H24) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human Interleukin-7 (IL-7).
应用说明（Application）
The Human Interleukin-7 (IL-7) ELISA Kit (Residue Testing) was developed for the detection and quantitative determination of GMP human IL-7 in samples from CAR-T product preparation processing where IL-7 is used as an immunotherapy agent.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
2-8℃
组分（Materials Provided）
IDComponentsSizeRES055-C01Pre-coated Anti-IL-7 Antibody Microplate1 plate(8×12 strips)RES055-C02Human IL-7 Standard20 μgRES055-C03Biotin-Anti-IL-7 Antibody20 μgRES055-C04Streptavidin-HRP50 μLRES055-C0510xWashing Buffer50 mLRES055-C062xDilution Buffer50 mLRES055-C07Substrate Solution12 mLRES055-C08Stop Solution7 mL原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of IL-7. The kit consists of Pre-coated Anti-IL-7 Antibody Microplate and Human IL-7 Standard and Biotin-Anti-IL-7 Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Human IL-7 as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-IL-7 Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
质量管理控制体系（QMS）
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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背景介绍
Interleukin 7 is also known as IL7, IL-7, and is a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells, but is not produced by lymphocytes. IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells, It also stimulates proliferation of all cells in the lymphoid lineage (B cells, T cells and NK cells). It is important for proliferation during certain stages of B-cell maturation, T and NK cell survival, development and homeostasis. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL-7 binds to the IL-7 receptor, a heterodimer consisting of Interleukin-7 receptor alpha and common gamma chain receptor. IL-7 promotes hematological malignacies (acute lymphoblastic leukemia, T cell lymphoma). Elevated levels of IL-7 have also been detected in the plasma of HIV-infected patients. IL-7 as an immunotherapy agent has been examined in many pre-clinical animal studies and more recently in human clinical trials for various malignancies and during HIV infection. IL-7 could also be beneficial in improving immune recovery after allogenic stem cell transplant.
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