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分子别名（Synonym）
TNFRSF9,4-1BB,CD137,CDw137,ILA
表达区间及表达系统（Source）
Biotinylated Human 4-1BB, His,Avitag (41B-H82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # Q07011-1).
Predicted N-terminus: Leu 24
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 20.9 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Biotinylated Human 4-1BB, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized UreluMab, Human IgG4 at 1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) with a linear range of 1-15 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5μg/well) plate can bind Human 4-1BB Ligand, Fc Tag with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Utomilumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) with a linear range of 0.5-8 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human 4-1BB Protein, His,Avitag (Cat. No. 41B-H82E6) captured on Biotin CAP-Series S sensor Chip can bind anti 4-1BB mAb with an affinity constant of 0.158 nM as determined in a SPR assay (Biacore T200) (Routinely tested) .
Protocol
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