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分子别名（Synonym）
Apolipoprotein E,APOE,Apo-E
表达区间及表达系统（Source）
Mouse Apolipoprotein E Protein, His Tag (APE-M52H5) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Gln 311 (Accession # P08226).
Predicted N-terminus: Glu 19
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.9 kDa. The protein migrates as 14 kDa and 35 kDa under reducing (R) condition (SDS-PAGE).
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Mouse Apolipoprotein E Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human LDL R, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) with a linear range of 0.02-2.5 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) immobilized on CM5 Chip can bind Mouse LDL R, His Tag (Cat. No. LDR-M52H8) with an affinity constant of 73.4 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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