产品详情
分子别名(Synonym)
TNFRSF17,CD269,BCM,BCMA
表达区间及表达系统(Source)
Human BCMA Protein, Llama IgG2b Fc Tag (BCA-H5259) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).
Predicted N-terminus: Met 1
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 33.7 kDa. The protein migrates as 40-47 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Unconjugated
内毒素(Endotoxin)
Less than 0.01 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
产品展示
电泳(SDS-PAGE)
Human BCMA Protein, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性(Bioactivity)-ELISA
Immobilized Human BAFF, His Tag at 5 μg/mL (100 μL/well) can bind Human BCMA Protein, Llama IgG2b Fc Tag (Cat. No. BCA-H5259) with a linear range of 0.02-0.4 ng/mL (QC tested).
ProtocolImmobilized Human BCMA Protein, Llama IgG2b Fc Tag (Cat. No. BCA-H5259) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA Mab with a linear range of 0.4-3 ng/mL (Routinely tested).
ProtocolImmobilized Human BCMA Protein, Llama IgG2b Fc Tag (Cat. No. BCA-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag (Cat. No. APL-H82F5) with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
用户评价 发表评论
