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分子别名（Synonym）
CC chemokine receptor 8,CD198,ChemR1,CKRL1,CKR-L1,CKRL1,CY6,GPRCL1,GPRCY6,GPR-CY6,TER-1
表达区间及表达系统（Source）
Human CCR8 Protein, Flag,His Tag&Twin-Strep Tag (CC8-H52D6) is expressed from human 293 cells (HEK293). It is a membrane protein, contains complete wild type 7-transmembrane domans (TMD) and extracellular doman (ECD).
蛋白结构（Molecular Characterization）
This protein carries a Flag tag at the N-terminus, and a polyhistidine tag at the C-terminus, followed by a twin strep tag. The protein has a calculated MW of 62.0 kDa.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of detergent.
Detergent buffer is INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove detergent.
Detergent buffer is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, Buffer B, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
- *The detergent Buffer B (Cat. No. LG-13) is sold separately and not included in protein, you can follow this link for product information.
产品展示
活性（Bioactivity）-ELISA
Immobilized Human CCR8 Protein, Flag,His Tag&Twin-Strep Tag (Cat. No. CC8-H52D6) at 0.5 μg/mL (100 μL/well) can bind Anti-CCR8 Antibody, Human IgG1 with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized Human CCR8 Protein, Flag,His Tag&Twin-Strep Tag (Cat. No. CC8-H52D6) at 5 μg/mL (100 μL/well) can bind Human CCL1, Fc Tag (Cat. No. CC1-H5254) with a linear range of 0.01-0.625 μg/mL (Routinely tested).
Protocol
Immobilized Human CCR8 Protein, Flag,His Tag&Twin-Strep Tag (Cat. No. CC8-H52D6) at 0.5 μg/mL (100 μL/well) can bind Anti-CCR8 Antibody with a linear range of 0.2-4 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-CCR8 captured on Protein A Chip can bind Human CCR8 Protein, Flag,His Tag&Twin-Strep Tag (Cat. No. CC8-H52D6) with an affinity constant of 63.8 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Protocol
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