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Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin

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CDD-C5259-100ug
¥3865.00
CDD-C5259-1mg (500ug X 2)
¥25475.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CD3E & CD3D,CD3 delta & CD3 epsilon

  • 表达区间及表达系统(Source)

    Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc (CDD-C5259) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5-1 (CD3E) & Q95LI8-1 (CD3D)).

    Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CD3E & CD3D Structure

    Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.9 kDa (CD3E) and 40.8 kDa (CD3D). Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 50 kDa and 55-65 kDa due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 0.01 EU per μg by the LAL method.

  • 纯度(Purity)

    90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    CD3E & CD3D SDS-PAGE

    Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%undefined.

  • 活性(Bioactivity)-ELISA

     CD3E & CD3D ELISA

    Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc (Cat. No. CDD-C5259) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human CD3 (SP34-2) (mouse IgG1) with a linear range of 2-63 ng/mL (QC tested).

    Protocol

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背景介绍

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

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