Biotinylated Human CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

分子别名（Synonym）

CD3 epsilon & CD3 gamma,CD3E & CD3G

表达区间及表达系统（Source）

Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (CDG-H82W3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1 (CD3E) & AAI13831 (CD3G)).

Predicted N-terminus: Asp 23 (CD3E)

蛋白结构（Molecular Characterization）

Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 18.6 kDa (CD3E) and 14.7 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 20-24 kDa and 25-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glycosylation.

偶联（Conjugate）

Biotin

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程