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分子别名（Synonym）
CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
表达区间及表达系统（Source）
Biotinylated Human CD40 Ligand Protein, Avitag,His,Flag Tag (CDL-H82Db) is expressed from human 293 cells (HEK293). It contains AA Gly 116 - Leu 261 (Accession # P29965-1).
Predicted N-terminus: Gly
蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by flag tag. The polyhistidine tag is inserted in-between the Avi tag and Flag tag.
The protein has a calculated MW of 51.6 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Biotinylated Human CD40 Ligand Protein, Avitag,His,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human CD40 Ligand Protein, Avitag,His,Flag Tag (Cat. No. CDL-H82Db) is more than 90% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CD40 Protein, Fc Tag (MALS verified) (Cat. No. CD0-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD40 Ligand Protein, Avitag,His,Flag Tag (Cat. No. CDL-H82Db) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human CD40 Ligand Protein, Avitag,His,Flag Tag (Cat. No. CDL-H82Db) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CD40 Protein, Fc Tag (Cat. No. CD0-H5253) with a linear range of 0.5-8 ng/mL (Routinely tested).
Protocol
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背景介绍
CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
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