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分子别名（Synonym）
CD47,MER6,IAP,OA3
表达区间及表达系统（Source）
Human CD47 Protein, Llama IgG2b Fc Tag (CD7-H5251) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3).
Predicted N-terminus: Gln 19
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 41.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human CD47 Protein, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human SIRP alpha, His Tag (Cat. No. SIA-H5225) at 0.5 μg/mL (100 μL/well) can bind Human CD47 Protein, Llama IgG2b Fc Tag (Cat. No. CD7-H5251) with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
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