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Biotinylated Rhesus macaque CD5 Protein, His,Avitag™ (MALS verified)

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货号-规格
价格
Qty.
CD5-R82E3-25ug
¥3865.00
CD5-R82E3-200ug
¥15215.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CD5,LEU1

  • 表达区间及表达系统(Source)

    Biotinylated Rhesus macaque CD5 Protein, His,Avitag (CD5-R82E3) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Thr 382 (Accession # F6V5Q9).

    Predicted N-terminus: Arg 25

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CD5 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 43 kDa. The protein migrates as 52-57 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Biotin

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    90% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    CD5 SDS-PAGE

    Biotinylated Rhesus macaque CD5 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD5 SEC-MALS

    The purity of Biotinylated Rhesus macaque CD5 Protein, His,Avitag (Cat. No. CD5-R82E3) is more than 90% and the molecular weight of this protein is around 85-105 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     CD5 ELISA

    Immobilized Biotinylated Rhesus macaque CD5 Protein, His,Avitag (Cat. No. CD5-R82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD5 Antibody, Mouse IgG1 (2C8A7) with a linear range of 0.2-8 ng/mL (QC tested).

    Protocol

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背景介绍

T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1,which is phosphorylated on tyrosine residues by LYN,so CD5 can create binding sites for PTPN6/SHP-1.CD5 may act as a receptor in regulating T-cell proliferation. CD5 is expressed at various developmental and activation stages on human B cells.CD5 is a well established negative regulator of TCR and BCR signalling.CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. Development,selection and function of different B- and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules.

前沿进展

 
药物研发进展
  • 英文全称:

    T-cell surface glycoprotein CD5

  • 中文全称:

    T细胞表面糖蛋白CD5

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    8 详情

  • 最高研发阶段:

    临床二期

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