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分子别名（Synonym）
CCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding protein 8
表达区间及表达系统（Source）
Mouse CTGF, His Tag (CTF-M52Hc) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Ala 348 (Accession # P29268-1).
Predicted N-terminus: Gln 26
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 37.3 kDa. The protein migrates as 37-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Potassium glutamate and Arginine, pH7.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Mouse CTGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Mouse CTGF, His Tag (Cat. No. CTF-M52Hc) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Monoclonal Anti-Human CTGF Antibody, Human IgG1 on AHC Biosensor, can bind Mouse CTGF, His Tag (Cat. No. CTF-M52Hc) with an affinity constant of 2.46 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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