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分子别名（Synonym）
Delta3,delta-like 3 (Drosophila),delta-like protein 3,DLL3,Pudgy,SCDO1,SCDO1delta3
表达区间及表达系统（Source）
Mouse DLL3, His Tag (DL3-M52H9) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Arg 488 (Accession # O88516-1).
Predicted N-terminus: Asp
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus and a fusion design at the N-terminus. The protein has a calculated MW of 118 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE).
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Mouse DLL3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.
SEC-MALS
The purity of Mouse DLL3, His Tag (Cat. No. DL3-M52H9) was greater than 85% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Mouse DLL3, His Tag (Cat. No. DL3-M52H9) at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding EGFs of DLL3), Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Mouse DLL3, His Tag (Cat. No. DL3-M52H9) at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding DSL of DLL3), Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Bispecific T-cell Engager captured on Protein A Chip can bind Mouse DLL3, His Tag (Cat. No. DL3-M52H9) with an affinity constant of 1.13 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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