Biotinylated Bovine FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (MALS & SPR verified)

分子别名（Synonym）

FcRn,FCGRT & B2M

表达区间及表达系统（Source）

MABSol® Biotinylated Bovine FcRn Heterodimer Protein, His,Avitag (FCN-B82W3) is expressed from human HEK293 cells. It contains AA Ala 24 - Pro 298 (FCGRT) & Ile 21 - Leu 118 (B2M) (Accession # Q3T119-1 (FCGRT) & P01888-1 (B2M)).

Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

蛋白结构（Molecular Characterization）

Biotinylated Bovine FcRn Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.5 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit B2M contains no tag. The reducing (R) protein migrates as 35-38 kDa (FCGRT) and 12 kDa (B2M) respectively due to glycosylation.

偶联（Conjugate）

Biotin

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程