产品详情
分子别名(Synonym)
Glucagon R, GCGR,Glucagon receptor
表达区间及表达系统(Source)
Biotinylated Human GCGR Protein, His,Avitag (GCR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Lys 136 (Accession # P47871-1).
Predicted N-terminus: Ala 26
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 16.6 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Biotin
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
数据展示
电泳(SDS-PAGE)
Biotinylated Human GCGR Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%undefined.
SEC-MALS
The purity of Biotinylated Human GCGR Protein, His,Avitag (Cat. No. GCR-H82E3) is more than 85% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-SPR
Biotinylated Human GCGR Protein, His,Avitag (Cat. No. GCR-H82E3) immobilized on CM5 Chip can bind GLP-1 (7-37) with an affinity constant of 48.5 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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