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分子别名（Synonym）
GM-CSF,CSF2,MGC131935
表达区间及表达系统（Source）
MABSol® Biotinylated Human GM-CSF, epitope tag free, primary amine labeling (GMF-H8214) is expressed from human HEK293 cells. It contains AA Ala 18 - Glu 144 (Accession # NP_000749.2). It is the biotinylated form of Human GM-CSF, premium grade (GMF-H4214).
Predicted N-terminus: Ala 18
Request for sequence蛋白结构（Molecular Characterization）
The product does NOT contain any epitope tags. The protein has a calculated MW of 14.5 kDa. The protein migrates as 18-28 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Biotinylated Human GM-CSF, epitope tag free, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human GM-CSF, epitope tag free, primary amine labeling (Cat. No. GMF-H8214) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.2 μg/well) plate can bind Human GM-CSF R alpha, Fc Tag (Cat. No. GRA-H5255) with a linear range of 1-8 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human GM-CSF, epitope tag free, primary amine labeling (Cat. No. GMF-H8214) on SA Biosensor, can bind Human GM-CSF R alpha, His Tag (SPR verified) (Cat. No. GRA-H52H7) with an affinity constant of 23.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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