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分子别名（Synonym）
MET,AUTS9,HGFR,RCCP2,c-Met
表达区间及表达系统（Source）
Biotinylated Human HGF R, Avitag,His Tag (MET-H82E1) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932 (Accession # P08581-1).
Predicted N-terminus: Glu 25 (α chain) & Ser 308 (β chain)
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 32.5 kDa (α chain) & 72.7 kDa (β chain). The protein migrates as 40-50 kDa (α subunit) and 90-100 kDa (β subunit) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Biotinylated Human HGF R, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human HGF R, Avitag,His Tag (Cat. No. MET-H82E1) is more than 85% and the molecular weight of this protein is around 110-140 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human HGF Protein, His Tag (Cat. No. HGF-H52H3) at 2 μg/mL (100 μL/well) can bind Biotinylated Human HGF R, Avitag,His Tag (Cat. No. MET-H82E1) with a linear range of 10-156 ng/mL (QC tested).
Protocol
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