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PE-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Tetramer Protein

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HLD-HP2H6-25ug
¥4160.00
HLD-HP2H6-200ug (100ug X 2)
¥23940.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    HLA-A*1101 & B2M & KRASG12D (VVVGADGVGK)

  • 表达区间及表达系统(Source)

    PE-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Tetramer Protein (HLD-HP2H6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGADGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGADGVGK).

    Predicted N-terminus: Gly 25 & Ile 21

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    PE-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Tetramer Protein is assembled by biotinylated monomer (HLD-H82E9) and PE-labeled streptavidin.

    Biotinylated Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12D peptide. Biotinylated Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • 偶联(Conjugate)

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 活性(Bioactivity)-ELISA

     HLA-A*1101 & B2M & KRASG12D (VVVGADGVGK) ELISA

    Immobilized PE-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Tetramer Protein (Cat. No. HLD-HP2H6) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol
  •  HLA-A*1101 & B2M & KRASG12D (VVVGADGVGK) ELISA

    Immobilized PE-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Tetramer Protein (Cat. No. HLD-HP2H6) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (Routinely tested).

    Protocol

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背景介绍

The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*1101 KRASG12D (VVVGADGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVVGADGVGK peptide of the KRASG12D.

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