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PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein

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HLU-HP2H5-25ug
¥4160.00
HLU-HP2H5-200ug (100ug X 2)
¥23940.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    HLA-E*0103 & B2M & CMV UL40 (VMAPRTVLL)

  • 表达区间及表达系统(Source)

    PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein (HLU-HP2H5) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMAPRTVLL peptide (Accession # P13747 (HLA-E*01:03) & P61769-1 (B2M) & VMAPRTVLL).

    Predicted N-terminus: Gly 22 & Val

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein is assembled by biotinylated monomer (HL0-H82E7) and PE-labeled streptavidin.

    Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Complex Protein is produced by co-expression of HLA and B2M loaded with CMV UL40 peptide. Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • 偶联(Conjugate)

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 活性(Bioactivity)-ELISA

     HLA-E*0103 & B2M & CMV UL40 (VMAPRTVLL) ELISA

    Immobilized Human NKG2C&CD94, Fc Tag (Cat. No. NC4-H5258) at 5 μg/mL (100 μL/well) can bind PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein (Cat. No. HLU-HP2H5) with a linear range of 0.01-5 μg/mL (QC tested).

    Protocol

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背景介绍

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

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