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分子别名（Synonym）
IL-15 R alpha,CD215,IL15RA,IL-15RA,IL-15R-alpha
表达区间及表达系统（Source）
Human IL-15 R alpha, Fc Tag (ILA-H5253) is expressed from human 293 cells (HEK293). It contains AA Ile 31 - Thr 205 (Accession # Q13261-1).
Predicted N-terminus: Ile 31
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 44.8 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human IL-15 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human IL-15 R alpha, Fc Tag (Cat. No. ILA-H5253) is more than 90% and the molecular weight of this protein is around 120-156 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-15 R alpha, Fc Tag (Cat. No. ILA-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15, Fc,Avitag (Cat. No. IL5-H82F3) with a linear range of 1-31 ng/mL (QC tested).
Protocol
活性（Bioactivity）-CELL BASE
Human IL-15 R alpha, Fc Tag (Cat. No. ILA-H5253) inhibits IL-15-dependent proliferation of CTLL-2 cells. The ED50 for this effect is 2.77-4.20 ng/mL (Routinely tested).
Protocol
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