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分子别名（Synonym）
IL2RB,RP5-1170K4.6,CD122,P70-75
表达区间及表达系统（Source）
Biotinylated Human IL-2 R beta, His,Avitag (ILB-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # P14784-1).
Predicted N-terminus: Ala 27
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 28.2 kDa. The protein migrates as 35-38 kDa and 42-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Biotinylated Human IL-2 R beta, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2 R beta, His,Avitag (Cat. No. ILB-H82E3) with a linear range of 0.005-1.25 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human IL-2 R beta, His,Avitag (Cat. No. ILB-H82E3) on SA Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Biotinylated Human IL-2 R beta, His,Avitag (Cat. No. ILB-H82E3) on SA Biosensor, can bind Human IL-15, premium grade (Cat. No. IL5-H4117) with an affinity constant of 16.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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背景介绍
CD122 is also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
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