Mouse Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein, His Tag&Tag Free (MALS verified)

分子别名（Synonym）

Integrin alpha 2 beta 1,ITGA2 & ITGB1

表达区间及表达系统（Source）

Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-M52W3) is expressed from human 293 cells (HEK293). It contains AA Tyr 27 - Thr 1129 (ITGA2) & Gln 21 - Asp 728 (ITGB1) (Accession # Q62469-1 (ITGA2) & P09055-1 (ITGB1)).

Predicted N-terminus: Tyr 27 (ITGA2) & Gln 21 (ITGB1)

蛋白结构（Molecular Characterization）

Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA2 and ITGB1, has a calculated MW of 127.6 kDa (ITGA2) and 83.5 kDa (ITGB1). Subunit ITGA2 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 140-150 kDa (ITGA2) and 100-120 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

偶联（Conjugate）

Unconjugated

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

90% as determined by SDS-PAGE.

90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程