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分子别名（Synonym）
Integrin alpha V beta 6,ITGAV&ITGB6
表达区间及表达系统（Source）
Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (IT6-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6) (Accession # NP_002201.1 (ITGAV) & AAI21179.1 (ITGB6)).
Predicted N-terminus: Phe 31 (ITGAV) & Gly 22 (ITGB6)
Request for sequence蛋白结构（Molecular Characterization）
Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 112.9 kDa (ITGAV) & 79.6 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The protein migrates as 85 kDa (ITGB6) and 140 kDa (ITGAV) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.
SEC-MALS
The purity of Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-H52E1) is more than 85% and the molecular weight of this protein is around 200-240 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-H52E1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.5-4 ng/mL (QC tested).
Protocol
Immobilized Human Fibronectin at 10 μg/mL (100 μL/well) can bind Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-H52E1) with a linear range of 0.313-2.5 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Fibronectin fragment, premium grade (Cat. No. FIN-H5113) immobilized on CM5 Chip can bind Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-H52E1) with an affinity constant of 1.23 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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