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分子别名（Synonym）
tumor necrosis factor receptor superfamily member 16; CD271; Gp80-LNGFR; member 16 nerve growth factor receptor; p75 ICD; p75 NTR
表达区间及表达系统（Source）
Human NGFR Protein, Fc Tag (NGR-H5254) is expressed from human 293 cells (HEK293). It contains AA Lys 29 - Asn 250 (Accession # P08138-1).
Predicted N-terminus: Lys 29
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 50.0 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human NGFR Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human NGFR Protein, Fc Tag (Cat. No. NGR-H5254) is more than 95% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-BLI
Loaded Human NGFR Protein, Fc Tag (Cat. No. NGR-H5254) on Protein A Biosensor, can bind Human NT-4, Tag Free (Cat. No. NT4-H5114) with an affinity constant of 1.64 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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