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分子别名（Synonym）
DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
表达区间及表达系统（Source）
Mouse TNF-alpha, His Tag (TNA-M52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804).
Predicted N-terminus: Leu 80
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.1 kDa. The protein migrates as 19-20 kDa and 23-24 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Mouse TNF-alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Mouse TNF-alpha, His Tag (Cat. No. TNA-M52H3) at 0.2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
Immobilized Mouse TNF-alpha, His Tag (Cat. No. TNA-M52H3) at 0.2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
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