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分子别名（Synonym）
FLT,VEGFR1,FLT1
表达区间及表达系统（Source）
Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag (VE1-M5256) is expressed from human 293 cells (HEK293). It contains AA Tyr 23 - Glu 759 (Accession # P35969-1).
Predicted N-terminus: Tyr 23
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 109.8 kDa. The protein migrates as 140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
85% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human VEGF165,Native (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag (Cat. No. VE1-M5256) with a linear range of 2-31 ng/mL (QC tested).
Protocol
Immobilized Human VEGF-B, His Tag (Cat. No. VE6-H5225) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag (Cat. No. VE1-M5256) with a linear range of 8-63 ng/mL (Routinely tested).
Protocol
Immobilized Mouse VEGF120, Tag Free (Cat. No. VE0-M4211) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag (Cat. No. VE1-M5256) with a linear range of 4-63 ng/mL (Routinely tested).
Protocol
Immobilized Mouse VEGF164, Tag Free (Cat. No. VE4-M4216) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag (Cat. No. VE1-M5256) with a linear range of 2-31 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Mouse VEGF164, His,Avitag (Cat. No. VE4-M82Q3) on SA Biosensor, can bind Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag (Cat. No. VE1-M5256) with an affinity constant of 42.4 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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